20,000
genes
x5
guides per gene
x500
replicates per gene & vector
Large-scale genomic perturbation by CRISPR and high-content cellular profiling are each well-established, but approaches to integrate the two have not been accessible until now. Cell by cell, Bifrost’s optical pooled screening platform matches rich image-based profiling data with thousands of CRISPR perturbations across millions of individual cells. These data enable deep genomic interpretation and integration with other available data, all without breaking the bank.”
– Paul Blainey, PhD
Scientific Founder.
Genome-scale Functional Genomics to Advance Discovery
Our next-generation, high-speed, microscopy-based platform performs genome-wide pooled screens of CRISPR-based perturbation libraries, dramatically expanding the depth and scope of current functional genomics approaches. Cells are interrogated phenotypically with high resolution microscopy using phase contrast and epi-fluorescent methods, followed by in situ sequencing of the guide RNA.
Bifrost’s unique combination of deep single-cell profiling via microscopy, chemistry and compute is 10-100 times faster than other methods. With this platform, scientists can understand how cellular systems and pathways work and interact with the many different genes that influence and regulate their behavior. This rich and enormous data set can be used to:
- Map disease pathways
- Identify novel drug targets
- Validate drug targets
- Reveal hidden gene functions
- Identify and explore drug candidate MOA
- Understand dependency of drug responses on genetic variants
- Uncover variation in cellular effects of competing drug candidates
- Explore efficacy of drug combinations
Large-scale genomic perturbation by CRISPR and high-content cellular profiling are each well-established, but approaches to integrate the two have not been accessible until now. Cell by cell, Bifrost’s optical pooled screening platform matches rich image-based profiling data with thousands of CRISPR perturbations across millions of individual cells. These data enable deep genomic interpretation and integration with other available data, all without breaking the bank.”
– Paul Blainey, PhD,
Scientific Founder
Genome-scale Functional Genomics to Advance Discovery
Our next-generation, high-speed, microscopy-based platform performs genome-wide pooled screens of CRISPR-based perturbation libraries, dramatically expanding the depth and scope of current functional genomics approaches. Cells are interrogated phenotypically with high resolution microscopy using phase contrast and epi-fluorescent methods, followed by in situ sequencing of the guide RNA.
Our next-generation, high-speed, microscopy-based platform performs genome-wide pooled screens of CRISPR-based perturbation libraries, dramatically expanding the depth and scope of current functional genomics approaches. Cells are interrogated phenotypically with high resolution microscopy using phase contrast and epi-fluorescent methods, followed by in situ sequencing of the guide RNA.
Our next-generation, high-speed, microscopy-based platform performs genome-wide pooled screens of CRISPR-based perturbation libraries, dramatically expanding the depth and scope of current functional genomics approaches. Cells are interrogated phenotypically with high resolution microscopy using phase contrast and epi-fluorescent methods, followed by in situ sequencing of the guide RNA.